multiple—laser zetaview ® f-nta nanoparticle tracking analyzers system  (Particle Metrix)

Journal: PLOS ONE

Article Title: Respiratory extracellular vesicle isolation optimization through proteomic profiling of equine samples and identification of candidates for cell-of-origin studies

doi: 10.1371/journal.pone.0315743

Figure Lengend Snippet: Extracellular vesicles (EVs) were isolated from horse bronchoalveolar lavage fluid (BALF) (n = 3 horses) following sample processing via centrifugation (gray boxes in “Sample Processing” panel) using four different EV isolation methods, resulting in a total of 12 samples. EVs were evaluated and characterized through nanoparticle tracking analysis (NTA), micro bicinchoninic acid (microBCA) protein assay, proteomics, and transmission electron microscopy (TEM). Figure created with biorender.com.

Article Snippet: EV samples from N = 3 horses per isolation method were evaluated using the Multiple—Laser ZetaView ® f-NTA Nanoparticle Tracking Analyzers System (Particle Metrix), including nine fractions per horse for each of the two size exclusion chromatography columns.

Techniques: Isolation, Centrifugation, Transmission Assay, Electron Microscopy

Journal: bioRxiv

Article Title: Effect of homotypic vs . heterotypic interactions on the cellular uptake of extracellular vesicles

doi: 10.1101/2023.10.23.563628

Figure Lengend Snippet:

Article Snippet: EV particle concentrations were determined on a multiple-laser ZetaView f-NTA Nanoparticle Tracking Analyzer (Particle Metrix Inc., Mebane, NC).

Techniques: Concentration Assay, Standard Deviation

Journal: Frontiers in Cellular Neuroscience

Article Title: Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood–Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model

doi: 10.3389/fncel.2022.841544

Figure Lengend Snippet: Isolation and characterization of serum exosomes. (A) Schematic representation of serum exosomes (Con-exo) isolated from rats. (B) Con-exo was photographed by transmission electron microscopy (TEM). Scale bar = 50 nm. (C) Representative immunoblotting images of exosomal markers such as CD9 and TSG101 in Con-exo. (D) Particle size of Con-exo was measured by nanoparticle tracing analysis.

Article Snippet: The size and concentration distribution profile of serum exosomes were analyzed using a Multiple-Laser ZetaView ® f-NTA Nanoparticle Tracking Analyzers (Particle Metrix, Germany).

Techniques: Isolation, Transmission Assay, Electron Microscopy, Western Blot